Welcome to MolDiag-PaCa
Principal investigator
 
Jens Peter von Kries, Ph.D.
Tel.: 0049-30-9406-2982
Fax:                           -2922
Mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Web: fmp-berlin.de/kries


Participants of the group:
Christoph Erdmann, TA
Tel.: 0049-30-9406-2909

The Screening-Unit will be engaged in the search for and experimental characterization of small molecules which decorate pancreatic tumor antigens. In vitro assay suitable for high-throughput screening of the small molecule libraries of the FMP will be established in pilot screens.

 

The Screening-Unit of the FMP serves the central compound library of the german initiative for Chemical Biology (for details see http://www.chembionet.de). The library has been designed after analysis of the world drug index for privileged subscaffolds and selection of a structural diversity set of 17.000 compounds, which represents the chemical space of the WDI. This unique library has been set up in collaboration of the FMP with ChemDiv. and co-financed by the Max-Delbrueck-Center (MDC,Berlin) and the Gesellschaft f?technologische Forschung (GBF, Braunschweig). The ChemBioNet library also contains additional 4.000 compounds spent by chemists of the network. Screening and chemical data are combined in the central database of the ChemBioNet. In total the compound libraries of the FMP comprise more then 40.000 small molecules within sublibraries of the NIH, the natural product pool of the Hans-Knoell-Institute (HKI, Jena) and compounds synthesized by the medicinal chemistry group of the FMP.

Furthermore the screening unit of the FMP will set up assays for targets provided or identified by the subprojects. In this specific collaboration the screening unit will serve as facility for the identification of compounds which decorate specific pancreatic tumor antigens by automated screens of  compound libraries.


ChemBioNet Library:

The compound collection (17951 compounds) has been designed after analysis of the "World Drug Index, WDI" for privileged substructures or scaffolds by the drug design and modeling group of the "Leibniz-Institut für Molekulare Pharmakologie". The library is placed on 384-well microtiter plates with compounds solubilized at 10 mM concentrations in DMSO (created by ChemDiv). The last two columns of each plate contain only DMSO for set up of controls on the assay plates.

The library contains also about 1000 compounds from the international LOPAC collection of annotated compounds (provided by Sigma Aldrich).

The library is shared with the Leibniz-Institut für Molekulare Pharmakologie (FMP, Berlin), the Helmholtz-Zentrum für Infektionsforschung (former GBF, Braunschweig), the Max-Delbr?ntrum (MDC, Berlin) and the Max-Planck-Institut für Molekulare Physiologie (MPI, Dortmund). The library is cofinanced by FMP, GBF and MDC.




ChemBioNet 384-well Format

Compounds sequentially placed from A1 to P1 (1-16) with increasing numbers from top to bottom and left to right columns. Columns 23 and 24 contain DMSO for set up of controls after transfer of compounds to test plates.

Note that the format of the fragment library (FMP57, 20.000) differs in position of DMSO loaded columns
(column 1 and column 24)!!!